Journal: Journal of Translational Medicine

Article Title: Sodium-myoinositol cotransporter-1 downstream of m6A methyltransferase WTAP exerts a potential carcinogenicity in diffuse large B-cell lymphoma progression

doi: 10.1186/s12967-025-07303-7

Figure Lengend Snippet: SMIT1 accelerates DLBCL cell-derived tumor xenograft growth in vivo. ( A ) Growth curves of tumor xenografts formed by U2932 cells bearing oeSMIT1 and shSMIT1. ( B ) Photographs of tumor xenografts on day 24 after implantation. ( C ) Tumor weight of xenografts on day 24 after implantation. ( D ) Western blot assay showing the expression of SMIT1 in tumors formed by U2932 cells. ( E ) IHC staining showing Ki67 expression in tumors formed by U2932 cells. ( F ) TUNEL staining in tumors formed by U2932 cells. Data are presented as mean ± SD. † p < 0.05, †† p < 0.01, and ††† p < 0.001

Article Snippet: Subsequently, the sections were incubated with anti-Ki67 polyclonal rabbit antibody (1:200 dilution, Proteintech Group Inc., Rosemont, IL, USA, 27309-1-AP) or anti-phospho-AKT-S473 monoclonal rabbit antibody (1:50 dilution, ABclonal, AP1208) at 4°C overnight, followed by the incubation of horseradish peroxidase (HRP)-conjugated goat anti-rabbit IgG secondary antibody (1:500 dilution, ThermoFisher SCIENTIFIC, Pittsburgh, PA, USA, 31460) for 1 h at 37°C.

Techniques: Derivative Assay, In Vivo, Western Blot, Expressing, Immunohistochemistry, TUNEL Assay, Staining

Journal: Nature Communications

Article Title: Dimeric copper peptide incorporated hydrogel for promoting diabetic wound healing

doi: 10.1038/s41467-025-61141-1

Figure Lengend Snippet: a Representative fluorescence images of Ki67 staining of HUVEC cells after different treatments (green: Ki67; blue: cell nucleus, Scale bars = 20 µm). b Quantitative analysis of Ki67+ cells. Data represented as Mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. c Relative cell viability of NIH/3T3 cells co-cultured with D-CuP solution and M-CuP solution for 24 h. Data represented as Mean ± SD (n = 4 independent replicates, ns not significant). Statistical significance was assessed using two-way ANOVA with Tukey’s multiple comparisons test. d Representative images of tube formation assay in HUVEC cells stained with calcein-AM (green) (Tube: yellow arrow, Scale bars = 400 µm). e Quantitative analysis of total tube length. Data represented as Mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. f The concentrations of VEGF were determined by ELISA. Data represented as Mean ± SD (n = 3 independent replicates). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. g Representative images of transwell migration assay of L929 cells (Scale bars = 200 µm). h Quantitative analysis of total tube length. Data represented as Mean ± SD (n = 3). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. i Images of L929 cells scratch experiment after incubating with D-CuP or M-CuP at different times (Scale bars = 100 μm). j Cell scratch healing rates at different times. Data represented as Mean ± SD (n = 3 independent replicates). Statistical significance was assessed using two-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.

Article Snippet: Cells were incubated with rabbit polyclonal antibody against Ki67 (1:500, Cat No. 28074-1-AP, Lot No. 00141310, Proteintech, China), followed by CoraLite488 goat anti-Rabbit IgG(H + L) secondary antibody (1:500, Cat No. SA00013-2, Lot No. 20000839, Proteintech, China).

Techniques: Fluorescence, Staining, Cell Culture, Tube Formation Assay, Enzyme-linked Immunosorbent Assay, Transwell Migration Assay

Journal: Nature Communications

Article Title: Dimeric copper peptide incorporated hydrogel for promoting diabetic wound healing

doi: 10.1038/s41467-025-61141-1

Figure Lengend Snippet: The concentrations of ( a ) IL-6, ( b ) IL-10, and d VEGF in the wound tissue were determined by ELISA on day 3. Data represented as Mean ± SD. (n = 3 independent replicates) Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. c Representative images of immunohistochemistry staining of TNF-α, VEGF, CD31, and Ki67 (Scale bars = 100 μm) on day 7 and Masson staining on day 12 (Scale bars = 500 μm). Quantitative analysis of immunohistochemical staining for ( e ) CD31 and ( f ) Ki67 on day 7. Data represented as Mean ± SD. (n = 3 independent replicates) Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. g Quantification of the collagen deposition in different groups on day 12. Data represented as Mean ± SD (n = 3 independent replicates). Statistical significance was assessed using one-way ANOVA with Tukey’s multiple comparisons test. Source data are provided as a Source Data file.

Article Snippet: Cells were incubated with rabbit polyclonal antibody against Ki67 (1:500, Cat No. 28074-1-AP, Lot No. 00141310, Proteintech, China), followed by CoraLite488 goat anti-Rabbit IgG(H + L) secondary antibody (1:500, Cat No. SA00013-2, Lot No. 20000839, Proteintech, China).

Techniques: Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Staining, Immunohistochemical staining